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Agenda IB
Murilo Vieira Geraldo
urrently focused on the investigation of the impact of non-coding RNAs, mainly microRNAs and long non coding RNAs, on the modulation of key signaling pathways and its contribution for development of cancer and human infertility, through the use of large scale platforms of gene expression, computational approaches and in vitro and in vivo functional analysis.
Carla Beatriz Collares Buzato
Our research group focuses on the study of cell-cell and cell-matrix interactions mediated by cell junctions and their role in tissue homeostasis, particularly of epithelial tissue. The current lines of research are: 1) to understand the mechanisms of regulation of intercellular junctions in vivo and in vitro models, 2) to investigate the importance of cell-cell contact mediated by intercellular junctions in the process of cell maturation and dysfunction pancreatic beta during development and in the context of type 2 diabetes mellitus (T2D), and 3) to study the role of the intestinal epithelial barrier mediated by the occlusion junction in the pathogenesis of T2D and its regulation by the microbiota and by short-chain fatty acids. We use in the laboratory a multidisciplinary approach that integrates tissue and functional biology, confocal laser scanning microscopy, electrophysiology, physiology of intercellular/transepithelial transport, and molecular biology (Western Blot and Real-Time PCR) to define fundamentals of the structure and function of cell junctions and understand the regulatory mechanisms of these structures in vivo and in vitro models. This research can contribute to our understanding of the pathophysiological function of junctions and support research aimed at determining ways and pathways for regulating these membrane specializations with possible application in the treatment of certain diseases associated with their dysfunction. (CNPq Group, Communication and Cellular Adhesion).