24 e 25 de julho - A short course in epigenomics

O Instituto de Biologia, em parceria com o Centre National De La Recherche Scientifique (CNRS) promove, nos dias 24 e 25 de julho de 2019, o Workshop A short course in epigenomics. O evento conta com palestra e mini curso ministrado pelo Prof. Dr. Christoph Grunau (CNRS).

O Workshop tem 40 vagas e é gratuito. Faça a sua inscrição até 22 de julho! TODAS AS VAGAS FORAM PREENCHIDAS!

O evento é organizado pela Professora Fernanda Janku Cabral, do Departamento de Biologia Animal do IB.

A short course in epigenomics

Understanding the complex interactions between the environment and the genotype that bring about the phenotype are one of the central objectives but also one of the major challenges in evolutionary biology. Things have been further complicated by the idea that heritable changes in reaction norms cannot only be produced by genetic variations, but that a certain part might be due to epigenetic variants. A lively scientific debate has emerged around the question whether epigenetic changes (alone) can have any importance in evolutionary processes. We argue that both, and potentially other heritable elements, are necessary to convey information through time.
Genome and epigenome, the latter we will define tentatively as information unit that allows for heritable changes in gene function that is not based on changes of the DNA sequence, are tightly interrelated and interdependent. Neither genomes nor epigenomes can exist alone and they necessarily interact with each other as elements of an inheritance system. We define here a system as a collection of units (elements) that are linked and that influence each other through action and feedback (interactions), and that bring about over time observable effects (process). Systems biology can be used as guide to identify interactions, interacting elements, strength of interaction, and the time course of interaction events in the generation of phenotypes. For operational reason, natural systems will need to be simplified, and systems biology allows for selecting elements and interactions that will have major effects from those that have minor. 
Through interaction with environmental cues this inheritance system produces over time, in a developmental process phenotypes that can be selected for. In this systems approach to inheritance, elements such as genome or epigenome can only be operationally defined. It is then not only their relative importance but also the strength of reciprocal interaction that drives the production of phenotypes. The art of the experimenter will be to find the best operational definition of all elements of the inheritance system at identical levels of resolution either through molecular approaches or pedigree studies, and to evaluate their relative contributions to phenotypic variance. While this can now be done in many biological systems, major challenges remain to precisely characterize the interactions between genome and epigenome. But once this will be done, rational concepts can be developed and used to manipulate the inheritance system.
The lecture will give an introduction into the science of epigenetics, briefly explain the molecular nature of the bearers of epigenetic information (DNA methylation, histone modifications, non-coding RNA, and the topography of the interphase nucleus), and introduce how next-generation sequencing based approaches can be used to describe the epigenotype and its relation to gene expression. Practical classes will use command-line free bioinformatics to analyse public epigenomics and transcriptomics datasets using the Galaxy interface.

24/07 - Auditório da Pós-graduação do Instituto de Biologia (Bloco O, térreo)
08:00 An introduction into Epigenetics, basic concepts and analysis
10:00 Coffee Break
10:20 Discussion Panel
12:00 Encerramento

25/07 - Sala LB06 (Bloco C, 1º Andar)
08:00 Linking transcriptomics and epigenomics
10:00 Coffee Break
10:20 Bioinformatics and Galaxy
12:00 Lunch Break
14:00 Bioinformatics and Galaxy
16:00 Coffee Break
16:20 Bioinformatics and Galaxy
18:00 Encerramento